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We applied annotated genomic data to extend this anecdotal analysis. By overlaying oxygen-requirement annotations on a phylogenetic tree along with pathway capabilities, we ask whether particular types of organisms (aerobes, anaerobes, or facultative anaerobes) tend to be capable of one pathway or another. We find that there is a clear and statistically significant correlation between a microbe’s glycolytic capabilities and its oxygen requirement (, ). Specifically, the prevalence of the ED pathway rises with an organism’s exposure to molecular oxygen: anaerobes use the EMP pathway almost exclusively (P P P

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